Journal: Cell

Article Title: Distinct Hippocampal Pathways Mediate Dissociable Roles of Context in Memory Retrieval

doi: 10.1016/j.cell.2016.09.051

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: N/A TTX Latoxan, Valence, France L8503 CNQX Tocris Bioscience, Bristol, UK 1045 R-CPP Tocris Bioscience, Bristol, UK 0247 QX-314 Tocris Bioscience, Bristol, UK 2313 Picrotoxin Sigma-Aldrich P1675 Fetal bovine serum (FBS) Hyclone SH30070.03 Critical Commercial Assays Rapid DNA Ligation Kit Roche 11 635 379 001 Plasmid DNA Purification MACHEREY-NAGEL N/A Endotoxin-free Plasmid DNA Purification MACHEREY-NAGEL N/A In-Fusion HD cloning kit Clontech 639649 Experimental Models: Cell Lines B7GG Callaway E., Salk Institute N/A BHK-EnVA Callaway E., Salk Institute N/A HEK293-TVA Young J., Salk Institute N/A HEK293T Callaway E., Salk Institute N/A Experimental Models: Organisms/Strains C57BL6/J Harlan Ltd N/A hCAR (transgenic expression of a truncated human receptor for CAV-Cre) Pettersson, S., Karolinska Institutet, Tallone et al., 2001 N/A LSL-R26 Tva-lacZ (Rosa-LSL-TVA, Cre-dependent TVA expression) Saur, D., Technische Universitat Munchen, Seidler et al., 2008 N/A GAD2 Cre Huang, Z. J., Cold Spring Harbor Laboratory, Taniguchi et al., 2011 N/A GAD2 Cre ::LSL-R26 Tva-lacZ (GAD2-Cre-TVA) This paper N/A C57BL6/J::hCAR (F1) This paper N/A Recombinant DNA pSADΔG-ArchT-GFP This paper N/A pAAV-EF1α-DIO-rabG This paper N/A pcDNA-B19N Callaway E., Salk Institute N/A pcDNA-B19P Callaway E., Salk Institute N/A pcDNA-B19L Callaway E., Salk Institute N/A pcDNA-B19G Callaway E., Salk Institute N/A pSADΔG-F3 Callaway E., Salk Institute N/A Sequence-Based Reagents NheI-covering forward primer for Rabies G (GGCCAAGCTAGCATGGTTCCTCAGGCTCTCCTGT) Sigma-Aldrich N/A AscI-covering reverse primer for Rabies G (TTAAGGCGCGCCTTACAGTCTGGTCTCACCCCC) Sigma-Aldrich N/A Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/ Fiji 1.48 http://fiji.sc/ TrackEM plugin in Fiji 1.48 Cardona A. and Saalfeld S. http://imagej.net/TrakEM2 Turboreg plugin in ImageJ Thévenaz et al., 1998 N/A GraphPad Prism GraphPad Software Inc http://www.graphpad.com/scientific-software/prism/ Imaris 7.3.1 Bitplane www.bitplane.com/imaris/imaris MATLAB The MathWorks, Inc. http://ch.mathworks.com/products/matlab IGOR Pro WaveMetrics https://www.wavemetrics.com/ Neuromatic plug-in for IGOR Pro Jason Rothman https://www.neuromatic.thinkrandom.com Zen softwares Zeiss http://www.zeiss.com/corporate/en_de/global/home.html pClamp 10 Axon instruments https://www.moleculardevices.com/ Other Optic fibers Thorlabs BFH48-200 Custom built optrode Lüthi A., FMI, Basel; Wolff et al., 2014 N/A Miniature epifluorescence microscope Inscopix nVista HD, version 2 Gradient index lens Inscopix GLP-0673 Blue (465 nm, 10 mW) and yellow (590 nm, 2.5 mW) LED with LED-driver LD-1 Plexon N/A Yellow laser (589 nm wavelength) CNI Lasers, China MBL589 Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Polymer, Plasmid Preparation, DNA Ligation, DNA Purification, Cloning, Transgenic Assay, Expressing, Sequencing, Software, Microscopy